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Autor:
Frank M. Raushel, Leisha S. Mullins
Publikováno v:
Journal of the American Chemical Society. 121:3803-3804
Ammonia can replace glutamine as the ultimate source for the nitrogen requirement in the reaction presented above.3 A chemical mechanism for the enzymatic transformation is presented below in Scheme 1. The first ATP phosphorylates bicarbonate to form
Publikováno v:
Journal of the American Chemical Society. 119:3853-3860
Recent kinetic measurements of the oxidation of linoleic acid by soybean lipoxygenase show that the thermal rate constants, kH ) 280 ((12) s -1 and kD ) 5.0 ((0.1) s -1 , are weakly temperature dependent within the temperature interval 30-50 °C. The
Publikováno v:
Journal of the American Chemical Society. 117:11619-11627
The detailed mechanism of DNA hydrolysis by enzymes is of significant current interest. One of the most important questions in this respect is the catalytic role of metal ions such as Mg{sup 2+}. Experimental evaluation of the catalytic effects of th