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Autor:
Qingjie Ji, Nannan Zhang, Yan Song, Yuzhong Wang, Tielin Hao, Yunfeng Chen, Fangzhen Shan, Ran Li
Publikováno v:
Journal of neuroscience methods. 366
Background Schwann cells (SCs) isolation is one of the basic techniques for study of peripheral nervous system and peripheral neuropathy. A combined and effective method of isolating SCs from sciatic nerves of newborn mice with high yield and purity
Autor:
Roberto Farina de Almeida, Fernada Urruth Fontella, Douglas José Marques, Maurício Schüler Nin, Lucas dos Reis Izolan, Felipe Borges Almeida, Rianne Remus Pulcinelli, Solange Bandiera, Mirna Bainy Leal, Rosane Gomez
Publikováno v:
Journal of Neuroscience Methods. 366:109412
Background The effects of tobacco smoke on the central nervous system are usually studied with isolated nicotine, ignoring other compounds present in cigarette smoke. The few studies that use in vivo whole-body cigarette smoke exposure are usually pe
Autor:
Amy McCaughey-Chapman, Bronwen Connor
Publikováno v:
Journal of Neuroscience Methods. 277:83-87
Background Organotypic brain slice cultures are a useful tool to study neurological function as they provide a more complex, 3-dimensional system than standard 2-dimensional in vitro cell cultures. New method Building on a previously developed mouse
Autor:
Neeru M. Sharma, Hong Zheng, Kaushik P. Patel, Robson Augusto Souza Dos Santos, Matthew C. Zimmerman, Frédéric Frézard, Marco Antônio Peliky Fontes, Gisele Cristiane Vaz
Publikováno v:
Journal of Neuroscience Methods. 273:55-63
Background Liposomes are concentric lipid vesicles that allow a sustained release of entrapped substances. GABA (γ-aminobutyric acid) is the most prevalent inhibitory neurotransmitter in the central nervous system. New method Using GABA-containing l
Autor:
Tessa Gordon, Michael P. Willand, David Scholl, Jennifer J. Zhang, Gregory H. Borschel, Joseph Catapano
Publikováno v:
Journal of Neuroscience Methods. 259:122-128
Background Retrograde labeling permits the investigation of the number, distribution and axonal projections of neurons in the peripheral nervous system. The well technique for labeling peripheral nerves consists of incubating the exposed peripheral n
Autor:
Peter Huettl, Jorge E. Quintero, David A. Price, Francois Pomerleau, Jason J. Burmeister, Greg A. Gerhardt
Publikováno v:
J Neurosci Methods
Background Although GABA is the major inhibitory neurotransmitter in the CNS, quantifying in vivo GABA levels has been challenging. The ability to co-monitor both GABA and the major excitatory neurotransmitter, glutamate, would be a powerful tool in
Autor:
Sachiko Kuramitsu, Yasuhito Uezono, Suzuro Hitomi, Motohiro Matoba, Kanako Miyano, Yojiro Ota, Kou Matsuo, Kentaro Ono, Kiyotoshi Inenaga, Yuji Seta, Nozomu Harano, Kiichiro Yamaguchi
Publikováno v:
Journal of Neuroscience Methods. 239:162-169
Background Stomatitis induces severe and painful hypersensitivity to pungency and physical contact during meals. Many studies have used anesthetized animals to examine evoked nociception in the oral mucosa, but no reports have used traditional behavi
Publikováno v:
Journal of neuroscience methods. 300
It is well documented that caffeine exacerbates the hyperthermia associated with acute exposure to 3,4-methylenedioxymethamphetamine (MDMA) in rats. Previous reports have also indicated that MDMA-related enhancement of dopamine release is exacerbated
Publikováno v:
Journal of neuroscience methods. 300
Microdialysis is a technique that allows access to the extracellular space of many tissues. For behavioural studies, microdialysis probes are inserted into the brain under anesthesia, but the experiment can be done in awake and freely moving animals.
Autor:
Ji-Geng Yan, Lin-Ling Zhang, Ziyi Wang, Hani S. Matloub, Yuhui Yan, Michael Agresti, John A. LoGiudice, James R. Sanger
Publikováno v:
Journal of Neuroscience Methods. 217:54-62
After a nerve injury, calcium concentration in the intra-nerve fiber drastically increases. The purpose of our study was to test an implantable micro-osmotic pump to deliver medications to accelerate calcium absorption, thereby greatly improving nerv