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Autor:
Corinne Moulon, Lawrence J. Stern, Elizabeth D. Mellins, Alka Patel, Philip J. Norris, Thomas O. Cameron, Lucy R. Wedderburn, Eric S. Rosenberg
Publikováno v:
Journal of Immunological Methods. 268:51-69
Class I MHC-peptide oligomers (MHC tetramers) have become popular reagents for the detection and characterization of antigen-specific CD8(+) T cells. Class II MHC proteins can be produced by expression in Escherichia coli followed by in vitro folding