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Publikováno v:
JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY. 36:344-348
The wsv477 gene from WSSV was cloned in pGEX-4T vector and expressed in Escherichia coli BL21.Then the wsv477 protein was induced and expressed at 37 ℃,and the purified fusion protein was used as antigen to immunize mice,subsequently the specific p