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Autor:
Elena B. Tikhonova, Lan Guan, Aaron C. Scarpa, Parameswaran Hariharan, Kelsey J. Markham, Satoshi Katsube
Publikováno v:
The Journal of Biological Chemistry
The melibiose permease of Salmonella typhimurium (MelBSt) catalyzes the stoichiometric symport of galactopyranoside with a cation (H+, Li+, or Na+) and is a prototype for Na+-coupled major facilitator superfamily (MFS) transporters presenting from ba
Autor:
Brianne E. Lewis, Timothy L. Stemmler, José M. Argüello, Christopher M. Sassetti, Subhalaxmi Nambi, Sarju J. Patel, Jarukit E. Long
Publikováno v:
Journal of Biological Chemistry. 291:11529-11539
Little is known about iron efflux transporters within bacterial systems. Recently, the participation of Bacillus subtilis PfeT, a P1B4-ATPase, in cytoplasmic Fe(2+) efflux has been proposed. We report here the distinct roles of mycobacterial P1B4-ATP
Autor:
Wahyu Indra Duwi Fanata, Thiyagarajan Thulasinathan, Seongha Park, Tesfaye Mengiste, Sang Yeol Lee, Rikno Harmoko, Jae-Min Lim, Hyun-Kyeong Seo, Ki Seong Ko, Nirmal Kumar Ramasamy, Jae Yong Yoo, Kyun Oh Lee
Publikováno v:
Journal of Biological Chemistry. 290:16560-16572
The most abundant N-glycan in plants is the paucimannosidic N-glycan with core β1,2-xylose and α1,3-fucose residues (Man3XylFuc(GlcNAc)2). Here, we report a mechanism in Arabidopsis thaliana that efficiently produces the largest N-glycan in plants.
Autor:
Akimasa Miyanaga, Yohei Katsuyama, Nobutaka Funa, Yasuo Ohnishi, Ken-ichi Miyazono, Sueharu Horinouchi, Masaru Tanokura, Ryutaro Satou, Hiroki Ozawa
Publikováno v:
Journal of Biological Chemistry. 288:34146-34157
Type III polyketide synthases (PKSs) show diverse cyclization specificity. We previously characterized two Azotobacter type III PKSs (ArsB and ArsC) with different cyclization specificity. ArsB and ArsC, which share a high sequence identity (71%), pr
Publikováno v:
Journal of Biological Chemistry. 287:16230-16237
Conjugated linolenic acids (CLNs), 18:3 Δ9,11,13, lack the methylene groups found between the double bonds of linolenic acid (18:3 Δ9,12,15). CLNs are produced by conjugase enzymes that are homologs of the oleate desaturases FAD2. The goal of this
Publikováno v:
Journal of Biological Chemistry. 284:36462-36472
The bidirectional [NiFe] hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 was purified to apparent homogeneity by a single affinity chromatography step using a Synechocystis mutant with a Strep-tag II fused to the C terminus of HoxF. To i
Publikováno v:
The Journal of Biological Chemistry
Salicylate (SA, 2-hydroxybenzoate) is a phytohormone best known for its role as a critical mediator of local and systemic plant defense responses. In response to pathogens such as Pseudomonas syringae, SA is synthesized and activates widespread gene
Publikováno v:
Journal of Biological Chemistry. 283:28767-28776
To elucidate the mechanism of bifurcated oxidation of quinol in the cytochrome bc1 complex, Rhodobacter sphaeroides mutants, H198N and H111N, lacking heme bL and heme bH, respectively, were constructed and characterized. Purified mutant complexes hav
Autor:
Thomas L. Poulos, Yergalem T. Meharenna, Kate E. Slessor, James J. De Voss, Sonia M. Cavaignac
Publikováno v:
Journal of Biological Chemistry. 283:10804-10812
Cytochrome P450cin (CYP176A1) is a bacterial P450 isolated from Citrobacter braakii that catalyzes the hydroxylation of cineole to (S)-6β-hydroxycineole. This initiates the biodegradation of cineole, enabling C. braakii to live on cineole as its sol
Autor:
Nathan Cobb, Justin P. Ridge, Craig Hemann, Alastair G. McEwan, Gregory A. Polsinelli, Russ Hille
Publikováno v:
Journal of Biological Chemistry. 282:35519-35529
Mutants of the active site residues Trp-116 and Tyr-114 of the molybdenum-containing Me(2)SO reductase from Rhodobacter capsulatus have been examined spectroscopically and kinetically. The Y114F mutant has an increased rate constant for oxygen atom t