Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Lester Packer"'
Publikováno v:
Journal of Bioenergetics. 6:21-25
In the presence of substrate and oxygen, aurovertin-inhibited rat-liver mitochondria incorporate 0.27±0.02 nanomoles of [35S] thiophosphate per mg protein into an acid-precipitable fraction. This incorporation is not prevented by uncouplers of oxida
Publikováno v:
Journal of Bioenergetics. 5:85-105
It is not known whether limitation of lifespan represents a programmed genetic event or is a result of environmental factors imposed by the conditions of culture. An investigation of the factors surrounding the limitedin vitro lifespan of human diplo
Autor:
Lester Packer, Charles W. Mehard
Publikováno v:
Journal of Bioenergetics. 6:151-160
This investigation confirms the presence of the inducible mixed function hydroxylase enzyme system in nuclear membranes. The cytochrome P-450 spectrum and demethylase activity, markers of the enzyme system, were used to define its localization to the
Autor:
Lester Packer, Klaus Brinkmann
Publikováno v:
Journal of Bioenergetics. 1:523-526
Studies of the temperature dependence of the energy of activation of rat liver mitochondrial respiration have revealed a linearity in the energy of activation between 25 to 41° with changes in slope observed at 20–25° and higher than 41°. Analys
Autor:
Lester Packer
Publikováno v:
Journal of Bioenergetics. 3:115-127
It is generally assumed that the high efficiency of electron transport and energy coupling in primary energy transducing membranes is the result of a special spatial arrangement of the interacting components within the membrane. Indeed, in recent yea
Publikováno v:
Journal of Bioenergetics. 3:539-552
1. Monothiophosphate was used as a probe for substrate level phosphorylation, mitochondrial oxidative phosphorylation, and membrane permeability. 2. Thiophosphate does not support ATP synthesis either in mitochondria or model enzymic reactions, conse
Publikováno v:
Journal of Bioenergetics. 2:305-316
It has been shown previously that intact rat liver mitochondria can be separated into two populations (designated B2 and B3) with mean buoyant densities of 1·184 and 1·216 respectively, by isopycnic sucrose density gradient centrifugation. A compar
Autor:
Lester Packer, John M. Wrigglesworth
Publikováno v:
Journal of Bioenergetics. 1:33-43
The pH-dependent changes in structure of submitochondrial vesicles prepared from rat liver have been investigated by a variety of structural “probes”. The main changes are: (a) the volume of the vesicles as assessed by electron microscopy and pac
Publikováno v:
Journal of Bioenergetics. 1:511-522
Some of the apparently anomalous findings made with the fluorescent probe 8-anilino-1-naphthalene sulfonic acid (ANS) have been reinvestigated using rat liver mitochondria. The results have been found compatible with current views on energy conservat
Autor:
Janet West, Lester Packer
Publikováno v:
Journal of bioenergetics. 1(4)
This investigation has shown the following hierarchy of chloroplast reactions which were susceptible to increasing glutaraldehyde concentration given for 6 min at 0° C. (1) Cyclic and non-cyclic photophosphorylation was completely inhibited by 100 m