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Publikováno v:
Journal of Bacteriology
In vitro culture media are being developed to understand how host site-specific nutrient profiles influence microbial pathogenicity and ecology. To mimic the cystic fibrosis (CF) lung environment, a variety of artificial sputum media (ASM) have been
Publikováno v:
Journal of Bacteriology.
Resistance in VanA-type vancomycin resistant Enterococcus faecium (VREfm) is due to an inducible gene cassette encoding seven proteins ( vanRSHAXYZ ). This provides for an alternative peptidoglycan (PG) biosynthesis pathway whereby d- Alanyl- d- Alan
Autor:
Diana M. Downs, Tomokazu Ito
Publikováno v:
J Bacteriol
Pyridoxal 5′-phosphate (PLP) is the biologically active form of vitamin B6 and an essential cofactor in all organisms. In Escherichia coli, PLP is synthesized via the deoxyxylulose 5-phosphate (DXP)-dependent pathway that includes seven enzymatic s
Publikováno v:
J Bacteriol
The intrinsic resistance of Pseudomonas aeruginosa to many antibiotics limits treatment options for pseudomonal infections. P. aeruginosa’s outer membrane is highly impermeable and decreases antibiotic entry into the cell. We used an unbiased high-
Autor:
Wim F. J. Vermaas, Christoph Trautner
Publikováno v:
Journal of Bacteriology. 195:5370-5380
Sll1951 is the surface layer (S-layer) protein of the cyanobacterium Synechocystis sp. strain PCC 6803. This large, hemolysin-like protein was found in the supernatant of a strain that was deficient in S-layer attachment. An sll1951 deletion mutation
Publikováno v:
Journal of Bacteriology. 194:813-826
Extracellular DNA acts as a cation chelator and induces the expression of antibiotic resistance genes regulated by Mg 2+ levels. Here we report the characterization of novel DNA-induced genes in Pseudomonas aeruginosa that are annotated as homologs o
Publikováno v:
Journal of Bacteriology. 193:5119-5129
Agrobacterium tumefaciens is a facultative phytopathogen that causes crown gall disease. For successful plant transformation A. tumefaciens requires the membrane lipid phosphatidylcholine (PC), which is produced via the methylation and the PC synthas
Publikováno v:
Journal of Bacteriology. 193:5668-5674
Purine nucleoside phosphorylase (PNP) is an important enzyme in purine metabolism and cleaves purine nucleosides to their respective bases. Mycobacterial PNP is specific for 6-oxopurines and cannot account for the adenosine (Ado) cleavage activity th
Publikováno v:
Journal of Bacteriology. 193:1815-1822
Previously, we showed that a 145-kb DNA region, including the entire kanamycin biosynthetic gene cluster (with two kanamycin resistance genes), was tandemly amplified up to 36-fold in an industrial strain of Streptomyces kanamyceticus. Strain improve
Autor:
Thomas A. Bobik, Shouqiang Cheng
Publikováno v:
Journal of Bacteriology. 192:5071-5080
Salmonella enterica degrades 1,2-propanediol (1,2-PD) in a coenzyme B 12 (adenosylcobalamin, AdoCbl)-dependent fashion. Salmonella obtains AdoCbl by assimilation of complex precursors, such as vitamin B 12 and hydroxocobalamin. Assimilation of these