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The Pol II preinitiation complex (PIC) influences Mediator binding but not promoter–enhancer looping
Publikováno v:
Genes Dev
Knowledge of how Mediator and TFIID cross-talk contributes to promoter–enhancer (P-E) communication is important for elucidating the mechanism of enhancer function. We conducted an shRNA knockdown screen in murine embryonic stem cells to identify t
Autor:
James Chuang, Magdalena Murawska, Dan Spatt, Dhawal Jain, Olga V. Viktorovskaya, Fred Winston, L. Stirling Churchman, Francheska Lopez-Rivera, Peter J. Park, Natalia I. Reim
Publikováno v:
Genes Dev
Histone chaperones are critical for controlling chromatin integrity during transcription, DNA replication, and DNA repair. Three conserved and essential chaperones, Spt6, Spn1/Iws1, and FACT, associate with elongating RNA polymerase II and interact w
Publikováno v:
Genes Dev
Transcriptionally silent genes must be activated throughout development. This requires nucleosomes be removed from promoters and enhancers to allow transcription factor (TF) binding and recruitment of coactivators and RNA polymerase II (Pol II). Spec
Autor:
Simon Labbé, Carlo Yague-Sanz, Maxime Duval, Marc Larochelle, Jude Beaudoin, Ronan Fernandez, Yann Vanrobaeys, Julien Berro, François Bachand, Pierre-Étienne Jacques
Publikováno v:
Genes Dev
Transcription by RNA polymerase II (RNAPII) is a dynamic process with frequent variations in the elongation rate. However, the physiological relevance of variations in RNAPII elongation kinetics has remained unclear. Here we show in yeast that a RNAP
Autor:
Marzia Munafò, Jonathan B. Preall, Benjamin Czech, Emma Kneuss, Evelyn L Eastwood, Undine-Sophie Deumer, Gregory J. Hannon
Publikováno v:
Genes & Development. 33:1208-1220
The PIWI-interacting RNA (piRNA) pathway is a conserved small RNA-based immune system that protects animal germ cell genomes from the harmful effects of transposon mobilization. In Drosophila ovaries, most piRNAs originate from dual-strand clusters,
Autor:
Karen Adelman, Leighton J. Core
Publikováno v:
Genes & Development. 33:960-982
Precise spatio–temporal control of gene activity is essential for organismal development, growth, and survival in a changing environment. Decisive steps in gene regulation involve the pausing of RNA polymerase II (Pol II) in early elongation, and t
Publikováno v:
Genes & Development. 32:1215-1225
Paused RNA polymerase II (Pol II) that piles up near most human promoters is the target of mechanisms that control entry into productive elongation. Whether paused Pol II is a stable or dynamic target remains unresolved. We report that most 5′ paus
Distinct patterns of histone acetyltransferase and Mediator deployment at yeast protein-coding genes
Publikováno v:
Genes & Development. 32:1252-1265
The transcriptional coactivators Mediator and two histone acetyltransferase (HAT) complexes, NuA4 and SAGA, play global roles in transcriptional activation. Here we explore the relative contributions of these factors to RNA polymerase II association
Publikováno v:
Genes & Development. 32:953-964
Packaging of pericentromeric DNA into heterochromatin is crucial for genome stability, development and health, yet its endogenous triggers remain poorly understood1. A defining feature of pericentromeric heterochromatin is histone H3 lysine 9 methyla
Autor:
Adam B. Burkholder, Karen Adelman, Christopher A. Lavender, Ginger W. Muse, David C. Fargo, Benjamin S. Scruggs, Telmo Henriques, Michiko O. Inouye, Lucy H. Williams
Publikováno v:
Genes & Development. 32:26-41
Regulation by gene-distal enhancers is critical for cell type-specific and condition-specific patterns of gene expression. Thus, to understand the basis of gene activity in a given cell type or tissue, we must identify the precise locations of enhanc