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Autor:
Shunsuke Kobayashi, Fumitaka Oyama, Vaclav Matoska, Shotaro Honda, Yasusato Sugahara, Akinori Kashimura, Peter O. Bauer, Minori Kamaya, Masayoshi Sakaguchi, Masahiro Kimura, Satoshi Wakita
Publikováno v:
FEBS Letters. 591:3310-3318
Mouse acidic mammalian chitinase (AMCase) degrades chitin with highest efficiency at pH 2.0 and is active up to pH 8.0. Here, we report that mouse AMCase also exhibits transglycosylation activity under neutral conditions. We incubated natural and art
Autor:
Jean Christophe Marvaud, Yannik Pereira, Mahmood Tavallaie, Maryse Gibert, Maria Manich, Stéphanie Raffestin, Alexandre Chenal, Daniel Gillet, Michel R. Popoff
Publikováno v:
FEBS Letters. 572:299-306
The botulinum neurotoxin A C-terminal fragment (Hc), which mediates the binding of the toxin to neuronal cell surface receptors, comprises two subdomains, Hc-N (amino acids 873–1095) and Hc-C (amino acids 1096–1296). In order to define the minima
Autor:
Wendy M. Macfarlane, Lucy J. Elrick, Helen Cragg, Kathleen I.J. Shennan, Kevin Docherty, Susan Campbell
Publikováno v:
FEBS Letters. 463:53-57
Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic β-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4. The other, termed Pax4c, was
Publikováno v:
FEBS Letters. 452:145-149
Triacylglycerols are the most important storage lipids in most plants and animals. Acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20) catalyzes the final step of the pathway of triacylglycerol synthesis and is the only step which is unique to this
Publikováno v:
FEBS Letters. 446:218-222
The present study demonstrates, in both hybrid NG108-15 and mouse neuroblastoma N18TG2 cells, the presence and regulation of PDE5 mRNA during cell differentiation. PDE5 cDNA probes in Northern blot analysis recognize a approximately 9 kb transcript i
Autor:
Takashi Kuwahara, Issei Tanaka, Akira Sugawara, Naohisa Tamura, Kei Tashiro, Masashi Mukoyama, Ken Ebihara, Yoshihiro Ogawa, S. Ozaki, Kazuwa Nakao, Tomohiro Aoki, Kiyoshi Mori
Publikováno v:
FEBS Letters. 417:371-374
Using the signal sequence trap method, we have cloned a novel 12-membrane-spanning transporter-like protein, termed renal-specific transporter (RST), from the mouse kidney. RST is a 553-amino-acid protein highly homologous to recently cloned organic
Publikováno v:
FEBS Letters. 416:312-316
cDNA cloning of glutamate transporter GLT-1 from mouse brain and liver has revealed that 5′-ends of the messages are different between brain and liver. In addition, one of the GLT-1 cDNAs isolated from liver has been found to possess a 3′-end dif
Publikováno v:
FEBS Letters. 414:33-38
The cDNAs for the two variable domains of the antibody 9E10 were cloned from the hybridoma cell line. A chimeric 9E10 Fab fragment was produced in E. coli under control of the tightly controlled tetracycline promoter. The functional Fab fragment was
Publikováno v:
FEBS Letters. 393:269-272
We have cloned and overexpressed rat 4-hydroxyphenylpyruvate dioxygenase (4HPPD) in Escherichia coli. The soluble, active recombinant enzyme was shown to contain both 4HPPD and α-ketoisocaproate dioxygenase (αKICD) activity. However, upon truncatio
Autor:
Marina V. Zinovjeva, Willem E. Fibbe, J. Mark J. M. Zijlmans, Alexander V. Belyavsky, Anna E. Popsueva, Jan W.M. Visser
Publikováno v:
FEBS Letters. 391:5-8
A novel cDNA encoding a putative secreted protein was isolated from murine bone marrow. The encoded protein named MCLP ( m urine c athelin- l ike p rotein) was found to be highly homologous to the pig cathelin, and to four neutrophil antimicrobial po