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Autor:
Anna Vaiopoulou, Emmanouil Liandris, Ioannis Kasampalidis, John Ikonomopoulos, Georgios Theodoropoulos, Styliani Taka, Pantelis Ntais, Maria Antoniou, Margarita Andreadou, Maria Gazouli
Publikováno v:
Experimental Parasitology. 131:419-424
Protozoa of the genus Leishmania are the causative agents of leishmaniosis. Although the polymerase chain reaction (PCR) has proved very effective in the detection of Leishmania DNA, a standardized method does not exist. In this study we attempt a co
Autor:
Fatemeh Mohtarami, Ahmad Ali Enayati, Ezatoldin Javadian, Alireza Sanei Dehkordi, Yavar Rassi, Zabiholah Zarei, Mohammad Ali Oshaghi, Mohammad Reza Abai
Publikováno v:
Experimental Parasitology. 131:344-349
Visceral leishmaniasis (VL) is an important health problem in Ardebil, where it borders Azerbaijan in the northwestern Iran. In spite of the presence of both cutaneous and visceral leishmaniasis (CL and VL) in northwestern Iran, previous researches h
Publikováno v:
Experimental Parasitology. 131:40-44
Loop-mediated isothermal amplification (LAMP) has been increasingly used for diagnosis and quantification of pathogens. Since the Bst DNA polymerase used in this assay is highly resistant to PCR inhibitors present in blood, direct analysis of blood s
Autor:
Jinliang Gao, Milin Ma, Qiaoyun Ren, Jianxun Luo, Guiquan Guan, Zhijie Liu, Aihong Liu, Youquan Li, Neil LeBlanc, Hong Yin, Qi Bai, Qinli Niu, Junlong Liu
Publikováno v:
Experimental Parasitology. 126:476-481
Theileria sergenti and Theileria sinensis are closely related members of benign Theileria species found in cattle and yaks in China. They are morphologically indistinguishable. A polymerase chain reaction (PCR) targeting major piroplasm surface prote
Autor:
Amir Ahmad Akhavan, H Abdoli, Jesus G. Valenzuela, Paul A. Bates, Reza Jafari, Mohammad Hossein Alimohammadian, Mohammad Reza Yaghoobi-Ershadi, Ali Khamesipour, Maryam Ghanei, Hossein Mirhendi, Mohammad Hossein Arandian, N. Shareghi, Niloufar Jalalizand, Shaden Kamhawi, Yavar Rassi
Publikováno v:
Experimental Parasitology. 126:552-556
Many rodent species act as reservoir hosts of zoonotic cutaneous leishmaniasis in endemic areas. In the present study a simple and reliable assay based on nested PCR was developed for the detection and identification of Leishmania parasites from rode
Autor:
Fernando Anaya-Velázquez, Minerva Paola Barrios-Ceballos, Claudia Leticia Mendoza-Macías, David Mirelman, Ángeles Rangel-Serrano, Felipe Padilla-Vaca, Lydia Patricia Cárdenas de la Peña
Publikováno v:
Experimental Parasitology. 121:167-174
Monoxenic cultivation of pathogenic Entamoeba histolytica trophozoites with Escherichia coli serotype 055 which binds strongly to the Gal/GalNAc amoebic lectin, markedly improved the growth of E. histolytica and produced a significant decrease in cys
Publikováno v:
Experimental Parasitology. 121:110-114
Strict regulation of gene expression is critical for the development of the malaria parasite within multiple host cell types. However, much remains unexplored regarding gene regulation in Plasmodium falciparum with only a few components of the gene r
Autor:
Trina Perrone, M. Mendoza, D. Fernández, B. González-Baradat, M.C. Eleizalde, Eglys González-Marcano
Publikováno v:
Experimental Parasitology. 121:1-7
Trypanosoma evansi is the causative agent of equine trypanosomosis, disease that affects horse's productivity and health. Parasitological and molecular methods are mostly used to detect the infection. The aim of this work was evaluate PCR sensitivity
Autor:
Meizhen Chen, Wei Zhou, Shoukun Wang, Cui-Qin Huang, Lingying Hu, Fuli Wen, Renfeng Chen, Liangping Yue
Publikováno v:
Experimental parasitology. 165
Isospora suis is an intestinal protozoan parasite in pigs. The 2-3 weeks old piglets are most often infected by I. suis because their immune system is not fully developed. The infection exhibits clinical features such as diarrhea and dehydration and
Publikováno v:
Experimental Parasitology. 110:303-308
Entamoeba histolytica and Entamoeba dispar are two morphologically indistinguishable species that are found in the human gut. Of the two, E. histolytica is considered to be pathogenic while E. dispar is nonpathogenic. To generate molecular probes to