Zobrazeno 1 - 10
of 83
pro vyhledávání: '"Daniel I. C. Wang"'
Autor:
Kristala L. J. Prather, James C. Leung, Magdy El-Said Mohamed, Daniel I. C. Wang, Andres Abin-Fuentes
Publikováno v:
Biotechnology and Bioengineering. 111:876-884
A mechanistic analysis of the various mass transport and kinetic steps in the microbial desulfurization of dibenzothiophene (DBT) by Rhodococcus erythropolis IGTS8 in a model biphasic (oil-water), small-scale system was performed. The biocatalyst was
Autor:
Daniel I. C. Wang, Mike Hoare, Douglas S. Clark, Juan A. Asenjo, Arnold L. Demain, Wei Shou Hu, Jonathan S. Dordick, Edwin N. Lightfoot, Harvey W. Blanch, Jerome S. Schultz, Christian Wandrey, Arthur E. Humphrey, Michael L. Shuler
Publikováno v:
Biotechnology and Bioengineering. 109:2417-2421
Autor:
Rui Tostoes, Daniel I. C. Wang, Paula M. Alves, Sofia B. Leite, Joana P. Miranda, Manuel J.T. Carrondo, Marcos F. Q. Sousa
Publikováno v:
Biotechnology and Bioengineering. 108:41-49
Long-term primary cultures of hepatocytes are essential for bioartificial liver (BAL) devices and to reduce and replace animal tests in lead candidate optimization in drug discovery and toxicology tests. The aim of this work was to improve bioreactor
Publikováno v:
Biotechnology and Bioengineering. 95:306-316
For the cultivation of mammalian cells on microcarriers a minimum inoculum concentration is required to initiate cell attachment and subsequent cell growth. A critical cell number model has been proposed to elucidate the mechanism of the inoculum req
Publikováno v:
Biotechnology and Bioengineering. 95:106-119
An important challenge facing therapeutic protein production in mammalian cell culture is the cleavage of terminal sialic acids on recombinant protein glycans by the glycosidase enzymes released by lysed cells into the supernatant. This undesired phe
Autor:
Adalberto Pessoa, Thereza Christina Vessoni Penna, Charles A. Haynes, Daniel I. C. Wang, Mojgan Kavoosi, Daniel Blankschtein, Henry H N Lam, Priscila Gava Mazzola
Publikováno v:
Biotechnology and Bioengineering. 93:998-1004
Green fluorescent protein (GFP) has been proposed as an ideal choice for a protein-based biological indicator for use in the validation of decontamination or disinfection treatments. In this article, we present a potentially scalable and cost-effecti
Publikováno v:
Biotechnology and Bioengineering. 93:1005-1016
Glycosylation engineering strategies that are currently used to improve quality of recombinant glycoproteins involve the manipulation of glycosyltransferase and/or glycosidase expression. We explored the possibility that over expressing nucleotide su
Autor:
Daniel Blankschtein, Adalberto Pessoa, Henry H N Lam, Daniel T. Kamei, Carlota de Oliveira Rangel-Yagui, Daniel I. C. Wang
Publikováno v:
Biotechnology and Bioengineering. 82:445-456
The enzyme glucose-6-phosphate dehydrogenase (G6PD) plays an important role in maintaining the level of NADPH and in producing pentose phosphates for nucleotide biosynthesis. It is also of great value as an analytical reagent, being used in various q
Publikováno v:
Biotechnology and Bioengineering. 85:177-184
The Chinese hamster ovary (CHO) cell line producing interferon-gamma (IFN-gamma) exhibits a 2-fold increase in specific productivity when grown at 32 degrees C compared to 37 degrees C. Low temperature also causes growth arrest, meaning that the cell
Autor:
Chia-li Liu, Daniel I. C. Wang, Daniel T. Kamei, Jonathan King, Daniel Blankschtein, Cameron Haase-Pettingell
Publikováno v:
Biotechnology and Bioengineering. 78:190-202
The partitioning behavior of viruses in the two-phase aqueous nonionic n-decyl tetra(ethylene oxide) (C10E4) micellar system cannot be fully explained by considering solely the repulsive, steric, excluded-volume interactions that operate between the