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Publikováno v:
Biochimica et biophysica acta. 1256(1)
Tissue pieces as well as isolated epithelial cells taken from visceral rat yolk sacs at the 18th day of gestation were able to synthesize and to secrete apo B containing lipoproteins floating in the density ranges of VLDL, IDL and LDL. In all three d
Publikováno v:
Biochimica et biophysica acta. 1243(3)
Mature adult rat hepatocytes were cultured as monolayers in serum-free Williams medium E containing 10(-7) M each of insulin (Ins), dexamethasone (Dex) and triiodothyronine (T3) and 30 mM pyruvate. The hepatocytes remained morphologically intact for
Publikováno v:
Biochimica et biophysica acta. 1255(3)
The effect of a range of dietary lipids on the fatty acid composition and membrane fluidity of lymphocytes was investigated. The effects of subsequent culture of these lymphocytes in medium containing autologous serum, foetal calf serum or a serum-fr
Publikováno v:
Biochimica et biophysica acta. 1255(1)
When triolein emulsions are enriched with cholesteryl oleate they are more readily removed by primary rat hepatocytes and HepG2 cells via an apolipoprotein E-responsive pathway. The increment in the cholesteryl ester does not appreciably change the s
Publikováno v:
Biochimica et biophysica acta. 1265(1)
The modulating effects of extracellular Mg2+ concentration ([Mg2+]o) on vascular contraction are well known. In the present study it was tested how the changes in cytosolic free Ca2+ concentration ([Ca2+]i) induced by various agonists are modified by
Publikováno v:
Biochimica et biophysica acta. 1224(3)
In hepatocytes in culture, ornithine aminotransferase activity remained higher when the cells were cultured at low oxygen tension (5%) than at high tension (21%), that is, it was higher in hepatovenous conditions. Northern blot analysis showed that t
Publikováno v:
Biochimica et biophysica acta. 1224(3)
The influence of androgenic status on basal and stimulated cAMP production, adenylyl cyclase activities and immunoblot quantified GS alpha and Gi alpha 2 subunits of the adenylyl cyclase regulatory proteins were compared in confluent preadipocytes fr
Publikováno v:
Biochimica et biophysica acta. 1224(1)
Previous quantification of glucocorticoid receptor (GR) binding in adipose tissue has been performed in cytosol preparations, which did not allow the determination of the total number of GR in the cell. Therefore, GR binding was determined in intact
Publikováno v:
Biochimica et biophysica acta. 1213(1)
Arachidonic acid (AA) metabolism, including mediator release and lipid turnover, was explored in [ 3 H]AA-radiolabelled alveolar macrophages obtained from guinea-pigs actively sensitized to ovalbumin (sAM) and controls (cAM). The basal and allergen-i
Publikováno v:
Biochimica et biophysica acta. 1220(3)
The effect of high and low pH on anoxic cell injury was studied in freshly isolated rat hepatocytes cast in agarose gel threads and perfused with Krebs-Henseleit bicarbonate buffer (KHB) saturated with 95% O2 and 5% CO2. Cytosolic free calcium (Ca2+i