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Publikováno v:
Biochimica et biophysica acta. 1259(1)
The oxidation, esterification and formation of chain elongated and desaturated products of [1-14C]5,8,11-eicosatrienoic (Mead) acid was studied. Liver cells from essentially fatty acid deficient (EFAD) and control rats were used. The metabolism of [1
Publikováno v:
Biochimica et biophysica acta. 1255(3)
The effect of a range of dietary lipids on the fatty acid composition and membrane fluidity of lymphocytes was investigated. The effects of subsequent culture of these lymphocytes in medium containing autologous serum, foetal calf serum or a serum-fr
Publikováno v:
Biochimica et biophysica acta. 1167(3)
The incorporation of [1-14C]16:0 and [1-14C]18:0 in the molecular species of PC and PE in isolated rat liver cells was studied. More [14C]18:0 than [14C]16:0 was esterified both in PC and PE. Also the chain elongated and desaturated products (16:1, 1
Publikováno v:
Biochimica et biophysica acta. 1124(2)
The influence of adrenergic and cholinergic mediators on phospholipid secretion in rat sublingual salivary gland cells maintained in the presence of [3H]choline was investigated. The secretion of [3H]choline-containing phospholipids over 30 min perio
Publikováno v:
Biochimica et biophysica acta. 1096(2)
Neutral lipid storage disease (NLSD) (Chanarin-Dorfman Syndrome) is an autosomal recessive disorder of multisystem triacyglycerol (TAG) storage. Previous work has pointed to a defect in intracellular TAG metabolism. In the studies reported here, the
Publikováno v:
Biochimica et biophysica acta. 1081(2)
(1) Rat and ovine hepatocytes were incubated in monolayer culture with various fatty acids to determine their effects on the composition of the lysophosphatidylcholine that was secreted. (2) No lysophosphatidylcholine was detected in the medium from
Publikováno v:
Biochimica et biophysica acta. 489(2)
Primary monolayer culture of adult rat hepatocytes represents a relatively novel and potentially useful in vitro technique for studying various hepatic processes for extended periods of time. This study evaluates the potential of hepatocyte monolayer
Autor:
William J. Longmore, Bruce D. Uhal
Publikováno v:
Biochimica et biophysica acta. 958(2)
Glycerol utilization for phospholipid biosynthesis was examined in type II pneumocytes isolated from normal and streptozocinin-diabetic rats. With glucose in the incubation medium, incorporation of exogenous [1,3-14C]glycerol into disaturated phospha
Publikováno v:
Biochimica et biophysica acta. 833(2)
There is evidence from whole animal and intact lung studies that prostaglandins are involved in the regulation of surfactant secretion. To explore this further we examined the effect of arachidonic acid on secretion of phosphatidylcholine in primary
Publikováno v:
Biochimica et biophysica acta. 878(3)
Glycerol 3-phosphate acylation was studied in type II cells isolated from adult rat lung. The process was found to be largely microsomal. In the microsomes phosphatidic acid is the main product of glycerol 3-phosphate acylation. Glycerol-3-phosphate