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Autor:
Munehiro Kitada, Keizo Kanasaki, Shin-ichi Tsuda, Ai Takeda-Watanabe, Shinji Kume, Daisuke Koya
Publikováno v:
Biochimica et biophysica acta. 1830(10)
Background Calorie restriction (CR) is accepted as an experimental anti-aging paradigm. Several important signal transduction pathways including AMPK and SIRT1 are implicated in the regulation of physiological processes of CR. However, the mechanisms
Autor:
Robert M. Sargis, Brian A. Neel, Yuxi Lin, Clifton O. Brock, Allison T. Hickey, Matthew J. Brady, Daniel A. Carlton
Publikováno v:
Biochimica et biophysica acta. 1822(6)
Emerging data suggest that environmental endocrine disrupting chemicals may contribute to the pathophysiology of obesity and diabetes. In a prior work, the phenylsulfamide fungicide tolylfluanid (TF) was shown to augment adipocyte differentiation, ye
Publikováno v:
Biochimica et biophysica acta. 1451(1)
Troglitazone is an oral insulin-sensitizing drug used to treat patients with type 2 diabetes. A major feature of this hyperglycemic state is the presence of increased rates of hepatic gluconeogenesis, which troglitazone is able to ameliorate. In this
Publikováno v:
Biochimica et biophysica acta. 1243(3)
Mature adult rat hepatocytes were cultured as monolayers in serum-free Williams medium E containing 10(-7) M each of insulin (Ins), dexamethasone (Dex) and triiodothyronine (T3) and 30 mM pyruvate. The hepatocytes remained morphologically intact for
Publikováno v:
Biochimica et biophysica acta. 1178(2)
In normal rat hepatocytes in primary culture the level of mRNA encoding the key gluconeogenic enzyme phospho enol pyruvate carboxykinase (PEPCK) is increased by the cyclic AMP analogue, chlorophenylthio cyclic AMP (cpt cAMP), and this response is rev
Autor:
Loranne Agius, Matthew Peak
Publikováno v:
Biochimica et biophysica acta. 1095(3)
The regulation of carbohydrate metabolism involves changes in the phosphorylation state of enzymes. We used okadaic acid, a potent inhibitor of protein phosphatases type 2A (IC50 0.05-2 nM) and type 1 (IC50 10–20 nM) to determine the role of these
Autor:
Philippe Legrand, André Bensadoun
Publikováno v:
Biochimica et biophysica acta. 1086(1)
Stearyl-CoA desaturase activity was measured as a function of time in culture in rat primary hepatocytes up to 90 h of culture. A decrease to half of the original activity occurred within 40 h of culture. Activity remained constant thereafter. Steary
Autor:
John N. Fain, Richard A. Pittner
Publikováno v:
Biochimica et biophysica acta. 1043(2)
Addition of vasopressin (100 nM) to rat hepatocytes prelabelled with [3H]inositol stimulated the production of inositol phosphates in the presence of 20 mM Li+. Preincubation of hepatocytes with insulin (50 nM) or glucagon (10 nM) had no significant
Publikováno v:
Biochimica et biophysica acta. 841(2)
In the present work we have been able to demonstrate that phenobarbital and lead exert an inducing effect on the biosynthesis of delta-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes of norma
Autor:
Christian Schudt
Publikováno v:
Biochimica et biophysica acta. 629(3)
A preparation method of monolayer cultures from rat hepatocytes with up to 95% plating efficiency is described. Cell number and DNA content per dish remained stable during 3 days in culture in hormone-free media containing 10 mM glucose. The cellular