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Publikováno v:
Biochimica et biophysica acta. 1829(1)
The eukaryotic RNA polymerase II (RNAPII) catalyzes the transcription of all protein encoding genes and is also responsible for the generation of small regulatory RNAs. RNAPII has evolved a unique domain composed of heptapeptide repeats with the cons
Autor:
Tuuli Kaambre, Kersti Tepp, Andrey V. Kuznetsov, Margus Eimre, Nathalie Beraud, Rita Guzun, Lumme Kadaja, Minna Varikmaa, Enn Seppet, Claire Monge, Natalja Timohhina, Valdur Saks
Publikováno v:
Biochimica et biophysica acta. 1797(6-7)
The aim of this review is to analyze the results of experimental research of mechanisms of regulation of mitochondrial respiration in cardiac and skeletal muscle cells in vivo obtained by using the permeabilized cell technique. Such an analysis in th
Publikováno v:
Biochimica et biophysica acta. 1767(9)
Since redox active metals are often transported across membranes into cells in the reduced state, we have investigated whether exogenous ferri-heme or heme bound to hemopexin (HPX), which delivers heme to cells via receptor-mediated endocytosis, inte
Autor:
Andrew Wilkins, Christopher L. Carpenter, Neil Justin, Vi Luan Ha, Borja Perez-Mansilla, Geraint M.H. Thomas
Publikováno v:
Biochimica et biophysica acta. 1761(12)
Phosphatidylinositol 4-phosphate 5-kinases [PtdIns4P5Ks] synthesise the majority of cellular phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P(2)] and phospholipase D1 (PLD1) synthesises large amounts of phosphatidic acid (PtdOH). The activities of
Autor:
Andrea Holt, Jean-Yves Lapointe, Michael J. Coady, Dominique G. Gagnon, Bernadette Wallendorff, Francis Bourgeois
Publikováno v:
Biochimica et biophysica acta. 1712(2)
The accessibility of the hydrophilic loop between putative transmembrane segments XIII and XIV of the Na+/glucose cotransporter (SGLT1) was studied in Xenopus oocytes, using the substituted cysteine accessibility method (SCAM) and fluorescent labelli
Publikováno v:
Biochimica et biophysica acta. 1712(1)
A chip-based biosensor technology using surface plasmon resonance (SPR) was developed for studying the interaction of ligands and G protein-coupled receptors (GPCRs). GPCRs, the fourth largest superfamily in the human genome, are the largest class of
Publikováno v:
Biochimica et biophysica acta. 1220(3)
Albumin and alpha-fetoprotein (AFP) are members of a multigene family which also includes vitamin-D-binding protein. Previous work in our laboratory has provided experimental support for the suggestion that the entry of unsaturated fatty acids into g
Autor:
D. Lockner, Tian-Xian Zhao
Publikováno v:
Biochimica et biophysica acta. 1153(2)
The electrical impedance of blood is primarily determined by plasma resistance R p , cell interior resistance R i , and cell membrane capacitance C m . These impedance parameters were measured for 62 samples with various erythrocytes sedimentation ra
Autor:
Jean Verdetti, Alain Queyroy
Publikováno v:
Biochimica et biophysica acta. 1108(2)
New methods are described to detect subconductance levels and to analyse ion channel gating. These methods are applied to simulated and experimental data. Single chloride channel records from inside-out membrane patches excised from human umbilical v
Publikováno v:
Biochimica et biophysica acta. 1070(2)
The transport of L-threonine was studied in cultured human fibroblasts. A kinetic analysis of L-threonine transport in a range of extracellular concentrations from 0.01 to 20 mM indicated that this amino acid enters cells through both Na(+)-independe