Zobrazeno 1 - 10
of 55
pro vyhledávání: '"Richard I"'
Publikováno v:
Biochemistry. 47:5406-5416
A long standing observation, that the photocycle of bacteriorhodopsin is markedly altered by the strength of actinic light, has not been adequately explained (1). The initial and primary illustration of the phenomenon is that the fraction of M-fast (
Publikováno v:
Biochemistry. 36:2439-2449
The single-turnover kinetics of the oxidation of cytochrome aa3 by O2 have been studied using a new approach. Up to 1000 whole spectra covering both the Soret and alpha regions were sequentially collected at room temperature from single samples with
Publikováno v:
Biochemistry. 35:5495-5508
We have investigated the role of purines in interstrand complex formation with regard to substitution of the negatively-charged, phosphodiester backbone by a nonionic, internucleoside linkage. Using the purine oligomer, d(AG)8, its methylphosphonate
Publikováno v:
Biochemistry. 34:13082-13090
The integration host factor (IHF) of Escherichia coli is a small, sequence-specific DNA-binding protein. The specific and nonspecific binding constants of IHF were estimated by gel-retardation assays. The equilibrium association constant of IHF for t
Autor:
Leonidas Phillips, Elizabeth J. Hindmarsh, Ronald G. Duggleby, Eve Szabados, Richard I. Christopherson
Publikováno v:
Biochemistry. 33:14237-14245
The bifunctional enzyme 5-aminoimidazole-4-carboxamide ribotide (AICAR) transformylase-IMP cyclohydrolase has been purified 780-fold to apparent homogeneity from human CCRF-CEM leukemia cells, completed with chromatography on Affi-Gel Blue followed b
Autor:
Anna Kobylanska, Carlos González, Wojciech J. Stec, Richard I. Hogrefe, Mark A. Reynolds, Thomas L. James
Publikováno v:
Biochemistry. 33:11062-11072
The solution structure of the thiophosphate-modified DNA.RNA hybrid duplex d(GCTATAApsTGG).r(CCAUUAUAGC) has been studied by NMR. Two samples with pure stereochemistry in the modified phosphate have been investigated. Two-dimensional NMR (2D NMR) met
Autor:
Richard I. Christopherson, Leonidas Phillips, Karl H. Rieckmann, Kristen K. Seymour, Stephen D. Lyons
Publikováno v:
Biochemistry. 33:5268-5274
The malarial parasite Plasmodium falciparum can only synthesize pyrimidine nucleotides via the de novo pathway which is therefore a suitable target for development of antimalarial drugs. New assay procedures have been developed using high-pressure li
Autor:
Thomas W. Traut, Hiu Chuen Lok, Maryam Shojaei, Camilla Chan, David B. Langley, Richard I. Christopherson, J. Mitchell Guss, Joel P. Mackay
Publikováno v:
Biochemistry. 47(12)
Orotidine 5'-monophosphate (OMP) decarboxylase from Plasmodium falciparum (PfODCase, EC 4.1.1.23) has been overexpressed, purified, subjected to kinetic and biochemical analysis, and crystallized. The native enzyme is a homodimer with a subunit molec
Publikováno v:
Biochemistry. 46(37)
Dihydroorotase (DHOase) catalyzes the reversible cyclization of N-carbamyl-l-aspartate (CA-asp) to l-dihydroorotate (DHO) in the de novo biosynthesis of pyrimidine nucleotides. Two different conformations of the surface loop (residues 105-115) were f
Autor:
Danny T. C. Huang, Richard I. Christopherson, Richard Wolfenden, R. Ian Menz, Feng Zhao, Vittorio L. Katis, Jacob Kaplan, R. Gerry Wake
Publikováno v:
Biochemistry. 45(27)
Dihydroorotase (DHOase, EC 3.5.2.3) from the extreme thermophile Bacillus caldolyticus has been subcloned, sequenced, expressed, and purified as a monomer. The catalytic properties of this thermophilic DHOase have been compared with another type I en