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Autor:
Gail Hilton, Ashwani Khanna, Jeannette Vasquez-Vivar, Vani Nilakantan, Galen M. Pieper, Nadine L. N. Halligan
Publikováno v:
Biochemical Journal. 391:541-547
Inducible nitric oxide synthase (iNOS) is a prominent component of the complex array of mediators in acute graft rejection. While NO production is determined by iNOS expression, BH4 (tetrahydrobiopterin), a cofactor of iNOS synthesized by GTP cyclohy
Publikováno v:
Biochemical Journal. 283:379-383
Fura-2-loaded human platelets were immobilized on a fibrinogen-coated surface and the cytosolic free calcium concentration ([Ca2+]i) was measured in single platelets by low-light-level video-ratio image-processing of the optical probe signal. Some fi
Publikováno v:
Biochemical Journal. 277:635-642
Interleukin 1 (IL1) increased phosphorylation of the small heat-shock protein (hsp 27) in MRC5 fibroblasts. The increase was maintained for at least 30 min, but levels had returned to pre-stimulation values by 2 h. When hsp 27 was metabolically label
Publikováno v:
Biochemical Journal. 269:831-834
Radioactive PtdIns(3)P was detected in human platelets incubated with [32P]Pi, but remained unaffected by thrombin treatment. In contrast, [32P]PtdIns(3,4)P2 was absent from resting platelets, but was produced by thrombin-activated platelets in a dos
Autor:
R M Palmer, P J Reeds
Publikováno v:
Biochemical Journal. 219:953-957
Forelimb digit extensor muscles from fed rabbits were incubated in the absence or presence of dexamethasone (100 nM). The presence of dexamethasone decreased the rates of protein synthesis, prostaglandin F2 alpha and prostaglandin E2 release after a
Publikováno v:
Biochemical Journal. 138:33-45
The transport and oxidation of glucose, the content of fructose 1,6-diphosphate, and the release of insulin were studied in microdissected pancreatic islets of ob/ob mice incubated in Krebs-Ringer bicarbonate medium. Under control conditions glucose
Autor:
S Guild, A H Drummond
Publikováno v:
Biochemical Journal. 221:789-796
The interaction between forskolin and vasoactive intestinal polypeptide (VIP) in the regulation of cyclic AMP production in GH3 pituitary tumour cells was investigated. Both forskolin (10nM-10 microns) and VIP (10pM-10nM) increased the cyclic AMP con
Publikováno v:
Biochemical Journal. 152:593-599
ADENOSINE (0.5 MM) added to hepatocyte suspensions increased the intracellular concentration of ATP and total adenine nucleotides within 60 min up to three-fold. 2. Adenosine at 0.5 mM inhibited gluconeogenesis from lactate by about 50%. At higher ad
Autor:
Robert H. Michell, Lynne M. Jones
Publikováno v:
Biochemical Journal. 142:583-590
When rat parotid fragments that had been labelled with32P in vivo were exposed to high concentrations of acetylcholine, radioactivity was lost from phosphatidylinositol but not from other phospholipids. Simultaneously the concentration of phosphatidy
Autor:
Kirsten Capito, C. J. Hedeskov
Publikováno v:
Biochemical Journal. 142:653-658
1. The concentration of cyclic AMP and the activity of phosphodiesterase were measured in isolated pancreatic islets from fed or 48h-starved mice. 2. Two different phosphodiesterases were detected. Neither the maximum activity nor the Km values of th