Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Michael J. MacDonald"'
Autor:
Luis A. Fernandez, James M. Ntambi, Israrul H. Ansari, Lucas M. O’Neill, Melissa J. Longacre, Michael J. MacDonald, Scott W. Stoker, Laura J. Zitur, Mindy A. Kendrick
Publikováno v:
Archives of Biochemistry and Biophysics. 618:32-43
Long-chain acyl-CoA synthetases (ACSLs) convert fatty acids to fatty acyl-CoAs to regulate various physiologic processes. We characterized the ACSL isoforms in a cell line of homogeneous rat beta cells (INS-1 832/13 cells) and human pancreatic islets
Autor:
Melissa J. Longacre, Siriluck Wattanavanitchakorn, Israr H. Ansari, Michael J. MacDonald, Scott W. Stoker, Mahmoud El Azzouny, Sarawut Jitrapakdee
Publikováno v:
Archives of biochemistry and biophysics. 676
Pyruvate carboxylase (PC) is an anaplerotic enzyme that supplies oxaloacetate to mitochondria enabling the maintenance of other metabolic intermediates consumed by cataplerosis. Using liquid chromatography mass spectrometry (LC-MS) to measure metabol
Sphingosine kinase 1 knockdown reduces insulin synthesis and secretion in a rat insulinoma cell line
Autor:
Scott W. Stoker, Suzanne G. Laychock, Mindy A. Kendrick, Noah R. Druckenbrod, Lucy D. Mastrandrea, Melissa J. Longacre, Michael J. MacDonald, Noaman Hasan
Publikováno v:
Archives of Biochemistry and Biophysics. 518:23-30
To evaluate the role of sphingosine kinase 1 (SphK1) in insulin secretion, we used stable transfection to knock down the expression of the Sphk1 gene in the rat insulinoma INS-1 832/13 cell line. Cell lines with lowered Sphk1 mRNA expression and SphK
Publikováno v:
Archives of Biochemistry and Biophysics. 488:100-104
Despite interest in malic enzyme(ME)s in insulin cells, mitochondrial malic enzyme (ME2) has only been studied with estimates of mRNA or with mRNA knockdown. Because an mRNA’s level does not necessarily reflect the level of its cognate enzyme, we d
Autor:
Laura J. Brown, Michael J. MacDonald
Publikováno v:
Archives of Biochemistry and Biophysics. 326:79-84
cDNAs which encode the rat testis and pancreatic islet mitochondrial glycerol phosphate dehydrogenase (mGPD) (EC 1.1.99.5), the key enzyme of the glycerol phosphate shuttle, were recently cloned and sequenced and found to contain calmodulin-like calc
Publikováno v:
Archives of Biochemistry and Biophysics. 279:104-108
Glyceraldehyde phosphate, a glycolytic intermediate, and succinic acid (as its methyl ester to make it permeable to the cell), a citric acid cycle intermediate, were the only glucose metabolites of many recently tested that stimulated insulin release
Publikováno v:
Archives of biochemistry and biophysics. 447(2)
Glyceraldehyde (GA) has been used to study insulin secretion for decades and it is widely assumed that beta-cell metabolism of GA after its phosphorylation by triokinase is similar to metabolism of glucose; that is metabolism through distal glycolysi
Publikováno v:
Archives of biochemistry and biophysics. 336(1)
A 2432-bp cDNA for mouse FAD-linked glycerol-3-phosphate dehydrogenase, a nuclear-encoded enzyme associated with the inner mitochondrial membrane, was isolated from a Lambda ZAP phage library generated from brown adipocyte mRNA. The amino acid sequen
Autor:
Michael J. MacDonald
Publikováno v:
Archives of biochemistry and biophysics. 305(2)
Pancreatic islets were cultured for 24 h in the presence of 1 mM glucose, which renders islets incapable of responding to glucose with insulin release. These islets were compared to islets maintained at 20 mM glucose for 24 h. Detritiation of [2-3H]g
Autor:
Michael J. MacDonald
Publikováno v:
Archives of biochemistry and biophysics. 300(1)
Previous work demonstrated that methyl esters of succinate are potent insulin secretagogues in pancreatic islets, while unesterified succinate is not. This can be explained by studies reported here, which show that 14C-labeled dimethyl succinate is m