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pro vyhledávání: '"James Travis"'
Publikováno v:
Analytical biochemistry. 260(2)
The need for a quick, simple screening method for the detection of general proteolytic activity prompted us to determine whether cleavage within the reactive site loop region (RSL) of alpha1-proteinase inhibitor (alpha1-PI), a well-characterized memb
Autor:
James Travis, David A. Johnson
Publikováno v:
Analytical Biochemistry. 72:573-576
A one-step purification of both human cationic trypsin and chymotrypsin I is described. The method involves the adsorption of the enzymes onto a Sepharose-Trasylol affinity column. Subsequent elution and separation of the two enzymes is accomplished
Publikováno v:
Analytical biochemistry. 89(1)
A simple and efficient procedure has been devised for the isolation of α-2-macroglobulin from human plasma (type 1-1 haptoglobulin). The primary step is gel filtration and affinity chromatography on Cibacron Blue Sepharose, which selectively removes