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Publikováno v:
Am J Physiol Cell Physiol
In vitro, cell cultures are essential tools in the study of intestinal function and disease. For the past few decades, monolayer cellular cultures, such as cancer cell lines or immortalized cell lines, have been widely applied in gastrointestinal res
Autor:
Oliver Speer, Anna Bogdanova, Lars Kaestner, Markus Schmugge, Andrea Brüggemann, Jeroen S. Goede, Max Gassmann, Asya Makhro, Pascal Hänggi, Jue Wang
Publikováno v:
American Journal of Physiology-Cell Physiology. 305:C1123-C1138
The presence of N-methyl-d-aspartate receptor (NMDAR) was previously shown in rat red blood cells (RBCs) and in a UT-7/Epo human myeloid cell line differentiating into erythroid lineage. Here we have characterized the subunit composition of the NMDAR
Autor:
Paul Canning, Alexander Zholos, F. De Courcey, Joseph Elborn, Mark Williams, Madeleine Ennis, H. L. Danahay, H. Atherton-Watson
Publikováno v:
American Journal of Physiology-Cell Physiology. 303:C1173-C1179
Cultured primary epithelial cells are used to examine inflammation in cystic fibrosis (CF). We describe a new human model system using cultured nasal brushings. Nasal brushings were obtained from 16 F508del homozygous patients and 11 healthy controls
Autor:
Gabriel C. Baltazar, Sonia Guha, Erin E. Coffey, Ronald C. Rubenstein, Alan M. Laties, Ji Liu, Claire H. Mitchell, Wennan Lu, William W. Reenstra
Publikováno v:
American Journal of Physiology-Cell Physiology. 303:C160-C169
The role of the cystic fibrosis transmembrane conductance regulator (CFTR) in lysosomal acidification has been difficult to determine. We demonstrate here that CFTR contributes more to the reacidification of lysosomes from an elevated pH than to base
Autor:
Pu Wang, Eric M. Andreoli, Wei Min Xu, Miguel R. Escobar, Rajesh Kher, Edward C. Sha, Robert L. Bacallao, Angela Wandinger-Ness
Publikováno v:
American Journal of Physiology-Cell Physiology. 301:C99-C105
While a variety of genetic mutations have been shown to be associated with renal cyst formation, mechanisms of renal cyst formation are largely unknown. In prior communications we described alterations in E-cadherin assembly in cultured cystic epithe
Publikováno v:
American Journal of Physiology-Cell Physiology. 295:C468-C474
Pancreatic β-cells are susceptible to oxidative stress, which is related closely to the islet dysfunction. In the present study, using the pancreatic cell lines HIT-T15 and RINm5F as known in vitro models of impaired β-cell function as well as prim
Autor:
Richard F. Loeser, Marcello DelCarlo
Publikováno v:
American Journal of Physiology-Cell Physiology. 290:C802-C811
Signals generated by the extracellular matrix (ECM) promote cell survival. We have shown that chondrocytes detached from their native ECM and plated without serum at low density on poly-l-lysine undergo significant cell death that is associated with
Publikováno v:
American Journal of Physiology-Cell Physiology. 283:C1675-C1686
Human umbilical vein endothelial cells (HUVECs) are an endothelial model of replicative senescence. Oxidative stress, possibly due to dysfunctional mitochondria, is believed to play a key role in replicative senescence and atherosclerosis, an age-rel
Publikováno v:
American Journal of Physiology-Cell Physiology. 283:C1548-C1556
PKC is a critical effector of plasma membrane dynamics, yet the mechanism and isoform-specific role of PKC are poorly understood. We recently showed that the phorbol ester PMA (100 nM) induces prompt activation of the novel isoform PKCepsilon followe
Publikováno v:
Scopus-Elsevier
The influence of zinc status on the levels of p53, as well as downstream targets of p53 in cell repair and survival, was examined in human aortic endothelial cells (HAECs). A serum-reduced low-zinc medium (ZD) was used to deplete zinc over one passag