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Publikováno v:
RNA. 28:263-273
This paper presents a probe comprising a fluorophore and a quencher, enabling measurement of released product from self-cleaving hammerhead ribozyme, without labeled RNA molecules, regular sampling or use of polyacrylamide gels. The probe is made of
Publikováno v:
Genome Research. 32:97-110
The correlation between codon and anticodon pools influences the efficiency of translation, but whether differences exist in these pools across individual cells is unknown. We determined that codon usage and amino acid demand are highly stable across
Publikováno v:
RNA
Exciting recent work has highlighted that numerous cellular compartments lack encapsulating lipid bilayers (often called “membraneless organelles”), and that their structure and function are central to the regulation of key biological processes,
Publikováno v:
RNA (New York, N.Y.), vol 27, iss 12
Understanding transcriptomes requires documenting the structures, modifications, and abundances of RNAs as well as their proximity to other molecules. The methods that make this possible depend critically on enzymes (including mutant derivatives) tha
Autor:
Bryan R. Cullen, Hal P. Bogerd, Cecilia Martínez-Campos, David G. Courtney, Christopher L. Holley, Kevin Tsai
Publikováno v:
RNA. 27:1400-1411
Pseudouridine ({psi}) is the most common non-canonical ribonucleoside present on mammalian non-coding RNAs (ncRNAs), including rRNAs, tRNAs and snRNAs, where it contributes ~7% of the total uridine level. However, {psi} constitutes only ~0.1% of the
Autor:
Hyeonju Jeong, Jungwook Kim
Publikováno v:
RNA. 27:1330-1338
During protein synthesis on ribosome, tRNA recognizes its cognate codon of mRNA through base-pairing with the anticodon. The 5′-end nucleotide of the anticodon is capable of wobble base-pairing, offering a molecular basis for codon degeneracy. The
Publikováno v:
Genome Res
Sequencing technologies using nucleotide conversion techniques such as cytosine to thymine in bisulfite-seq and thymine to cytosine in SLAM seq are powerful tools to explore the chemical intricacies of cellular processes. To date, no one has develope
Publikováno v:
RNA. 27:876-890
Mammalian C-to-U RNA editing was described more than 30 yr ago as a single nucleotide modification in small intestinal Apob RNA, later shown to be mediated by the RNA-specific cytidine deaminase APOBEC1. Reports of other examples of C-to-U RNA editin
Autor:
Anna A. Shalybkova, Ivan V. Kulakovskiy, Darya S. Mikhailova, Liliia I. Fakhranurova, Eugene F. Baulin
Publikováno v:
RNA. 27:907-919
Noncoding RNAs play a crucial role in various cellular processes in living organisms, and RNA functions heavily depend on molecule structures composed of stems, loops, and various tertiary motifs. Among those, the most frequent are A-minor interactio
Publikováno v:
RNA. 27:616-627
The enzyme Tpt1 is an essential agent of fungal tRNA splicing that removes an internal RNA 2′-PO4 generated by fungal tRNA ligase. Tpt1 performs a two-step reaction in which: (i) the 2′-PO4 attacks NAD+ to form an RNA-2′-phospho-(ADP-ribose) in