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Autor:
Rebecca MacLeod, Fok Vun Chan, Han Yuan, Xin Ye, Yun Jin Ashley Sin, Teraesa M. Vitelli, Tudor Cucu, Annie Leung, Irene Baljak, Samantha Osinski, Yuhong Fu, Gyu Ik Daniel Jung, Anant Amar, Paul L. DeAngelis, Urban Hellman, Mary K. Cowman
Publikováno v:
Analytical Biochemistry. 652:114769
A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nu
Autor:
Kimihiko Sugaya
Publikováno v:
Analytical biochemistry. 633
Epigenetics is thought to be involved in highly advanced life phenomena, and its regulation has created new opportunities in regenerative medicine. Mintbody (modification-specific intracellular antibody) can track a posttranslational protein modifica
Autor:
Michael S. Becker, Chantal Fuerstner, Laura Theisen, Raymond C. Harris, Frank Wunder, Manuel Grundmann, Florian Kölling, Celia Dobersalske, Andreas Timmermann
Publikováno v:
Analytical biochemistry. 630
Autotaxin (ATX) plays an important role in (patho-)physiological lysophosphatidic acid (LPA) signaling. Here we describe the establishment of novel cell-based ATX assay formats. ATX-mediated LPA generation is detected by using a stable LPA receptor r
Publikováno v:
Analytical Biochemistry. 571:25-36
Monoclonal antibody (mAb), one of the major types of therapeutic proteins in the pharmaceutical industry, is predominantly manufactured using mammalian cell culture [1]. Oxidative stress, potentially present during cell culture process, may increase
Autor:
Hamed Abiri, Farnoosh Attari, Mona Salimi, Fatemeh Farokhmanesh, Milad Gharooni, Alireza Alikhani, Hassan Moghtaderi, Mohammad Abdolahad
Publikováno v:
Analytical Biochemistry. :1-10
One of the most interesting fields of research in cancer diagnosis is tracing the relation between extracellular media and cancer progression. Detecting the secreting contents of the cells and translating these molecular identifications into label-fr
Publikováno v:
Analytical biochemistry. 628
Cancer is the second leading cause of death worldwide and most of the cancer-related deaths result from metastasis. As expressed on the surface of various cancer cell types, intercellular adhesion molecule-1 (ICAM-1) has been shown to play a role in
Publikováno v:
Analytical biochemistry. 597
Nitroxides and nitroxide-containing nanoparticles (RNP) are excellent antioxidants. However, they have relatively high reduction potentials, which make them behave like oxidants or show little activity in some antioxidant assays. We found that stable
Publikováno v:
Analytical Biochemistry. 552:75-80
Interest in the recently discovered phenomenon of mitochondrial transfer between mammalian cells has gained momentum since it was first described in cell culture systems more than a decade ago. Mitochondria-targeting fluorescent dyes have been repurp
Publikováno v:
Analytical biochemistry. 591
The ubiquitously expressed transglutaminase 2 (TG2) has diverse functions in virtually all cell types, with its role depending not only on cell type, but also on specific subcellular localization. In the central nervous system (CNS) different types o
Autor:
Lukas Schuster, Philipp Uhl, Andreas D. Meid, Max Sauter, Walter E. Haefeli, Dirk Theile, Johanna Weiss
Publikováno v:
Analytical biochemistry. 596
Quantification of therapeutic antibodies is commonly based on physico-chemical assays such as enzyme-linked immunoabsorption assays (ELISA) and lately on mass spectrometry. However, the functional integrity of evaluated immunoglobulins is yet not ass